SEPARATION OF REBAUDIOSIDE A FROM Stevia rebaudiana EXTRACT USING LOW POLARITY RESIN AB-8
Keywords:sweetener , Stevia rebaudiana , rebaudioside A , Steviol glycoside , adsorption separation method , macroporous resin
There are many methods to separate or purify the rebaudioside A compound from Stevia rebaudiana extract. However, the ion-exchange chromatography using macroporous resin is still the most popular among those methods. The separation of rebaudioside A from stevia crude extract by macroporous resin AB-8 was optimised in this adsorption separation study. This approach was applied to evaluate the influence of four factors such as the adsorption temperature, desorption time, elution solution ratio, and adsorption volume on rebaudioside A yield of the purified stevia extract. The
results showed that the low polarity resin AB-8 is able to separate rebaudioside A from stevia extract with 0.601 in yield compared to the high polarity resin HPD 600 with 0.204 in yield used in Anvari and Khayati study. The best conditions for rebaudioside A separation by macroporous resin AB-8 were at 35°C of adsorption temperature, 30 min of desorption time, elution solution ratio 2:1, and 50 mL of adsorption volume.
Anvari, M., & Khayati, G. (2016). Separation and purification of rebaudioside A from extract of Stevia Rebaudiana leaves by macroporous adsorption resins. Polish Journal of Chemical Technology, 18 (1), 127 – 132.
Babic, K., Van der Ham, L., & De Haan, A. B. (2006). Recovery of benzaldehyde from aqueous streams using extractant impregnated resins. Reactive and Functional Polymers, 66, 1494 – 1505.
Chatsudthipong, V., & Muanprasat, C. (2009). Stevioside and related compounds: Therapeutic benefits beyond sweetness. Pharmacology & Therapeutics, 21, 41 – 54.
Fu, X. C., Shen, W. X., & Yao, T. Y. (1990). Physical chemistry. Beijing: Higher Education Press, pp.172.
Geuns, J. M. C. (2003). Molecules of interest stevioside. Phytochemistry, 64, 913 – 921.
Liu, C., Li, L. S., Xu, L. L., & Zhou, Z. M. (2007). Separation and identification of stevioside and Rebaudiodside A in Stevia by HPLC. Chinese Journal of Medical Laboratory, 26, 23 – 26.
Liu, G. M., Zheng, S. R., Yin, D. Q., Xu, Z. Y., Fan, J., & Jiang, F. (2006). Adsorption of aqueous alkylphenol ethoxylate surfactants by mesoporous carbon CMK-3. Journal of Colloid and Interface Science, 302, 47– 53.
Sorana, D. B., & Lorentz, J. (2007). Design of experiments: Useful orthogonal arrays for number of experiments from 4 to 16. Entropy, 9, 198 – 232.
Wheeler, A., Boileau, A. C., Winkler, P. C., Compton, J. C., Prakash, I., Jiang, X., & Mandarino, D. A. (2008). Pharmacokinetics of rebaudioside A and stevioside after single oral doses in healthy men. Food and Chemical Toxicology, 46, 54 – 60.
Zhang, Y., Chen, T. H., Shi, Z. Q., & He, B. L. (1998). Studies on the separation of rebaudioside A by recrystallization. Ion Exchange and Adsorption, 14, 515 – 520.