SEPARATION OF REBAUDIOSIDE A FROM Stevia rebaudiana EXTRACT USING LOW POLARITY RESIN AB-8

Chong Saw Peng; Norazlina  Noordin; Mustapha  Akil; Norellia  Bahari

doi:10.51200/bijb.v1i.1546

Authors

Chong Saw Peng Agrotechnology and Bioscience Division, Malaysian Nuclear Agency (Nuclear Malaysia), Ministry of Energy, Science, Technology, Environment and Climate Change (MESTECC), Bangi, Selangor, Malaysia
Norazlina Noordin Agrotechnology and Bioscience Division, Malaysian Nuclear Agency (Nuclear Malaysia), Ministry of Energy, Science, Technology, Environment and Climate Change (MESTECC), Bangi, Selangor, Malaysi
Mustapha Akil Agrotechnology and Bioscience Division, Malaysian Nuclear Agency (Nuclear Malaysia), Ministry of Energy, Science, Technology, Environment and Climate Change (MESTECC), Bangi, Selangor, Malaysia
Norellia Bahari Agrotechnology and Bioscience Division, Malaysian Nuclear Agency (Nuclear Malaysia), Ministry of Energy, Science, Technology, Environment and Climate Change (MESTECC), Bangi, Selangor, Malaysia

DOI:

https://doi.org/10.51200/bijb.v1i.1546

Keywords:

sweetener , Stevia rebaudiana , rebaudioside A , Steviol glycoside , adsorption separation method , macroporous resin

Abstract

There are many methods to separate or purify the rebaudioside A compound from Stevia rebaudiana extract. However, the ion-exchange chromatography using macroporous resin is still the most popular among those methods. The separation of rebaudioside A from stevia crude extract by macroporous resin AB-8 was optimised in this adsorption separation study. This approach was applied to evaluate the influence of four factors such as the adsorption temperature, desorption time, elution solution ratio, and adsorption volume on rebaudioside A yield of the purified stevia extract. The
results showed that the low polarity resin AB-8 is able to separate rebaudioside A from stevia extract with 0.601 in yield compared to the high polarity resin HPD 600 with 0.204 in yield used in Anvari and Khayati study. The best conditions for rebaudioside A separation by macroporous resin AB-8 were at 35°C of adsorption temperature, 30 min of desorption time, elution solution ratio 2:1, and 50 mL of adsorption volume.

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