CHARACTERISATION OF AN ANTARCTIC YEAST, Glaciozyma antarctica PI12

Authors

  • Teoh Chul Peng Biotechnology Research Institute, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia
  • Koh Soon Peng Biotechnology Research Institute, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia
  • Clemente Michael Wong Vui Ling Biotechnology Research Institute, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia

DOI:

https://doi.org/10.51200/bijb.vi.2154

Keywords:

cell morphologies, bipolar budding, yeast chain, quiescence state, hydrolytic enzyme, Antarctic yeast, Glaciozyma spp, enzyme activities, psychrophiles

Abstract

Glaciozyma antarctica PI12 is a psychrophilic yeast isolated from Antarctica. It has an optimal growth in yeast peptone dextrose (YPD) and yeast mould (YM) broth media but not in potato dextrose (PD) broth medium. Early phase G. antarctica PI12 cells had elongated-shape and became oval-shaped as they aged. G. antarctica PI12 exhibited bipolar budding and formed a chain of cells during the lag and early exponential phases. The number of chains decreased as the yeast aged. It appeared mainly as a single cell at the stationary phase, and a small number of them still produced buds. Some cells at the stationary phase entered the quiescence state (G0) as a longterm survival strategy. The G. antarctica PI12 cell size decreased when they entered the stationary phase. G. antarctica PI12 was found to produce hydrolytic enzymes, chitinase, cellulase, mannanase, and xylanase. A higher glucose concentration of 2% in the PD agar medium inhibited the activities of chitinase but not the cellulase, mananase and xylanase.

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Published

2020-12-23

How to Cite

Teoh Chul Peng, Koh Soon Peng, & Clemente Michael Wong Vui Ling. (2020). CHARACTERISATION OF AN ANTARCTIC YEAST, Glaciozyma antarctica PI12. Borneo International Journal of Biotechnology (BIJB), 89 - 102. https://doi.org/10.51200/bijb.vi.2154