Analyzing Cymbopogon sp methanolic extracts via liquid chromatography
Keywords:
Chromatography, Cymbopogon, serai makan, lemongrass, monograph.Abstract
Cymbopogon species can be utilized in various ways. It is applied in cooking, aromatherapy, among others. The essential oil is also used extensively in traditional medicine. From the literatures, it possesses various classes of compounds, for example, terpenoids and flavonoids. In this study, an unidentified Cymbopogon methanolic was subjected to a Reversed Phased-High Performance Liquid Chromatography (RP-HPLC). The mobile phase was adapted from the national herbal monograph, comprising of 0.3% formic acid and acetonitrile. The HPLC chromatograms were recorded, and two major peaks were observed. Both signals were apparent in the stepwise gradient, when compared to the fast mode. In the absence of the standard monoterpenoids, geraniol, geranial and neral could be further investigated from this sample. In conclusion, a specimen of C. citratus could be involved in the experimental procedures.
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