Phytochemical screening and wound healing effect of Alocasia longiloba Miq. petiole ethanolic extract and its fractions in excision wound rat model
DOI:
https://doi.org/10.51200/bijb.v2i.4203Keywords:
Alocasia longiloba Miq., petiole extract, excision, wound, Sprague-Dawley rat, histologyAbstract
Alocasia longiloba Miq., or ‘keladi candik’ is traditionally used for wound healing in Malaysia. Preliminary studies have been performed to determine the dose range of A. longiloba for potential wound healing. Therefore, this study continues to determinethe phytochemical screening of fractions and their ability in accelerating wound healing. Ethanol crude extract of A. longiloba and its fractions of hexane, chloroform, and ethyl acetate was prepared and used for phytochemical screening and wound healing formulation. Seven groups of Sprague-Dawley rats (n = 3) were experimentally wounded in the dorsal area for 12 days. Group I was left untreated, Group II was dressed in petroleum jelly as the vehicle group, Group III was treated with Solcoseryl jelly 10% as the positive drug, and the rest of the groups were dressed with respective formulations at 6% concentration. The wound area was photographed and measured, and the histological examinations of granulation tissues were examined. The phytochemical screening of chloroform fraction shows an abundance of alkaloids, flavonoids, and tannins, which are considered responsible for wound healing activity. The chloroform fraction proved more effective, showing a significant percentage of wound contraction (p<0.05) compared to the negative and vehicle control groups. Moreover, histological observation of wounds treated with chloroform fraction of A. longiloba showed an organized epithelial layer, fewer blood vessels, and dense collagen fibres comparable with normal rat skin.
References
Abdul Latif, M., Mohd Zaki, M. Z., Tai, M. L., Abdul Rahman, N. H., Arshad, S. A., & Hammid, A.
(2015). Alocasia denudata Engler treatment enhance open wound healing activities in
Wistar rat’s skin. Journal of Ethnopharmacology, 176, 258 – 267. https://doi.org/10.1016/j.
jep.2015.10.036
Abdulhafiz, F., Mohammed, A., Kayat, F., Bhaskar, M., Hamzah, Z., Podapati, S. K., & Reddy, L.
V. (2020). Xanthine oxidase inhibitory activity, chemical composition, antioxidant
properties and GC-MS analysis of Keladi Candik (Alocasia longiloba Miq). Molecules, 25
(11), 2658. https://doi.org/10.3390/molecules25112658
Abubakar, A. R., & Haque, M. (2020). Preparation of medicinal plants: Basic extraction and
fractionation procedures for experimental purposes. Journal of Pharmacy and Bioallied
Sciences, 12 (1), 1 – 10. https://doi.org/10.4103/jpbs.JPBS_175_19
Adewumi, S. S., Akinpelu, B. A., Akinpelu, D. A., Aiyegoro, O. A., Alayande, K. A., & Agunbiade,
M. O. (2020). Studies on wound healing potentials of the leaf extract of Terminalia
avicennioides (Guill. & parr.) on wistar rats. South African Journal of Botany, 133, 285 – 297. https://doi.org/10.1016/j.sajb.2020.07.039
Ahmad, S. U., Aladdin, N. A., Jamal, J. A., Shuid, A. N., & Mohamed, I. N. (2021). Evaluation of woundhealing
and antioxidant effects of Marantodes pumilum (Blume) Kuntze in an excision wound model. Molecules, 26 (1), 228. https://doi.org/10.3390/molecules26010228
Anima, P., Arun, M., & Satish, S. (2019). Scientific validation of wound healing potential of
Jasminum sambac Ait. South African Journal of Botany, 121, 584 – 589. https://doi.org/10.1016/j.sajb.2018.11.018
Arbain, D., Sinaga, L. M. R., Taher, M., Susanti, D., Zakaria, Z. A., & Khotib, J. (2022). Traditional
uses, phytochemistry and biological activities of alocasia species: A systematic review.
Frontiers in Pharmacology, 13, 1 – 27. https://doi.org/10.3389/fphar.2022.849704
Asumang, P., Boakye, Y. D., Agana, T. A., Yakubu, J., Entsie, P., Akanwariwiak,, W. G., Adu, F., & Agyare, C. (2021). Antimicrobial, antioxidant and wound healing activities of methanol leaf extract of Bridelia micrantha (Hochst.) Baill. Scientific African, 14, e00980. https://doi.org/10.1016/j.sciaf.2021.e00980
Begashaw, B., Mishra, B., Tsegaw, A., & Shewamene, Z. (2017). Methanol leaves extract Hibiscus micranthus Linn exhibited antibacterial and wound healing activities. BMC Complementary and Alternative Medicine, 17, 1 – 11. https://doi.org/10.1186/s12906-017-1841-x
Campelo, M. B. D., Santos, J. D. A. F., Filho, A. L. M. M., Ferreira, D. C. L., Sant’Anna, L. B., de Oliveira, R. A., Maia, L. F., & Arisawa, E. A. L. (2018). Effects of the application of the amniotic membrane in the healing process of skin wounds in rats. Acta Cirurgica Brasileira, 33
(2), 144 – 155. https://doi.org/10.1590/s0102-865020180020000006
Che Hamzah, N. H., Arifullah, M., Sirajudeen, K. N. S., Asari, M. A., Hamzah, Z., & Shaik, I. K. (2019).
Keladi candik (Alocasia longiloba Miq.) petiole extracts promote wound healing in a full
thickness excision wound model in rats. Asian Pacific Journal of Tropical Biomedicine, 9
(4), 140 – 149. https://doi.org/10.4103/2221-1691.256727
Cheng, K. Y., Lin, Z. H., Cheng, Y. P., Chiu, H. Y., Wu, T. K., & Wu, J. S. (2018). Wound healing in
streptozotocin-induced diabetic rats using atmospheric-pressure argon plasma jet.
Scientific Reports, 8, 12214. https://doi.org/10.1038/s41598-018-30597-1
DiPietro, L. A. (2016). Angiogenesis and wound repair: When enough is enough. Journal of
Leukocyte Biology, 100, 979 – 84. https://doi.org/10.1189/jlb.4mr0316-102r
Do, Q. D., Angkawijaya, A. E., Tran-Nguyen, P. L., Huynh, L. H., Soetaredjo, F. E., Ismadji, S., & Ju, Y.
H. (2014). Effect of extraction solvent on total phenol content, total flavonoid content,
and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22
(3), 296 – 302. https://doi.org/10.1016/j.jfda.2013.11.001
Dorai, A. A. (2012). Wound care with traditional, complementary and alternative medicine. Indian
Journal of Plastic Surgery, 45 (2), 418 – 424. https://doi.org/10.4103/0970-0358.101331
Geow, C. H., Tan, M. C., Yeap, S. P., & Chin, N. L. (2021). A review on extraction techniques and its
future applications in industry. European Journal of Lipid Science and Technology, 123
(4), 1 – 10. https://doi.org/10.1002/ejlt.202000302
Gonzalez, A. C. D. O., Costa, T. F., Andrade, Z. D. A., & Medrado, A. R. A. P. (2016). Wound healing
– A literature review. Anais Brasileiros de Dermatologia, 91 (5), 614 – 620. https://doi.
org/10.1590/abd1806-4841.20164741
Häkkinen, L., Larjava, H., & Koivisto, L. (2011). Granulation tissue formation and remodeling.
Endodontic Topics, 24 (1), 94 – 129. https://doi.org/10.1111/etp.12008
Hossain, M. A., Al-Raqmi, K. A. S., Al-Mijizy, Z. H., Weli, A. M., & Al-Riyami, Q. (2013). Study of total
phenol, flavonoids contents and phytochemical screening of various leaves crude
extracts of locally grown Thymus vulgaris. Asian Pacific Journal of Tropical Biomedicine,
(9), 705 – 710. https://doi.org/10.1016/S2221-1691(13)60142-2
Imran, H., Ahmad, M., Rahman, A., Yaqeen, Z., Sohail, T., Fatima, N., Iqbal, W., & Yaqeen, S. S. (2015).
Evaluation of wound healing effects between Salvadora persica ointment and solcoseryl
jelly in animal model. Pakistan Journal of Pharmaceutical Sciences, 28 (5), 1777 – 1780.
Johnson, K. E., & Wilgus, T. A. (2014). Vascular endothelial growth factor and angiogenesis in the
regulation of cutaneous wound repair. Advances in Wound Care, 3, 647 – 661. https://
doi.org/10.1089/wound.2013.0517
Kothari, M., & Jain, D. K. (2017). Evaluation of wound healing activity and preliminary
phytochemical screening of Thespesia populnea bark extracts. Pharmaceutical and
Biosciences Journal, 5 (6), 48 – 55. https://doi.org/10.20510/ukjpb/5/i6/173520
Loh, E. Y. X., Mohamad, N., Fauzi, M. B., Ng, M. H., Ng, S. F., & Mohd Amin, M. C. I. (2018). Development
of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and
fibroblasts for full-thickness wound healing. Scientific Reports, 8, 2875. https://doi.
org/10.1038/s41598-018-21174-7
María, R., Shirley, M., Xavier, C., Jaime, S., David, V., Rosa, S., & Jodie, D. (2018). Preliminary
phytochemical screening, total phenolic content and antibacterial activity of thirteen
native species from Guayas province Ecuador. Journal of King Saud University – Science,
(4), 500 – 505. https://doi.org/10.1016/j.jksus.2017.03.009
Martinez, K. B., Mackert, J. D., & McIntosh, M. K. (2017). Polyphenols and intestinal health. In
Nutrition and functional foods for healthy aging (pp. 191 – 210). https://doi.org/10.1016/
B978-0-12-805376-8.00018-6
Minutti, C. M., Knipper, J. A., Allen, J. E., & Zais, D. M. W. (2017). Tissue-specific contribution of
macrophages to wound healing. Seminars in Cell and Developmental Biology, 61, 3 – 11.
https://doi.org/10.1016/j.semcdb.2016.08.006
Moghadamtousi, S. Z., Rouhollahi, E., Hajrezaie, M., Karimian, H., Abdulla, M. A., & Kadir, H. A.
(2015). Annona muricata leaves accelerate wound healing in rats via involvement of
Hsp70 and antioxidant defence. International Journal of Surgery, 18, 110 – 117. https://
doi.org/10.1016/j.ijsu.2015.03.026
Monika, P., Chandraprabha, M. N., Rangarajan, A., Waiker, P. V., & Murthy, K. N. C. (2022). Challenges
in healing wound: Role of complementary and alternative medicine. Frontiers in
Nutrition, 8, 1 – 13. https://doi.org/10.3389/fnut.2021.791899
Naik, V. K. M., Babu, K. S., & Latha, J. (2014). Preliminary phytochemical investigation and wound
healing activity of cardiospermum Halicacabum Linn. Scientific Reviews and Chemical
Communications, 4 (3), 101 – 108.
Nejjari, R., Benabbes, M., Amrani, M., Meddah, B., Bouatia, M., & Taoufik, J. (2019). Phytochemical
screening and wound healing activity of Telephium imperati (L.) in rats. South African
Journal of Botany, 123, 147 – 151. https://doi.org/10.1016/j.sajb.2019.03.023
Nur-Izzati, M., Arifullah, M., Nazahatul, A. A., Klaiklay, S., Chumkaew, P., Norhazlini, M. Z., Abdulhafiz,
F., & Zulhazman, H. (2021). Elucidation of total phenolic content and antioxidant activity
in medicinal aroid, Alocasia longiloba Miq. IOP Conference Series: Earth and Environmental
Science, 756, 012043. https://doi.org/10.1088/1755-1315/756/1/012043
Olugbuyiro, J. A. O., Abo, K. A, & Leigh, O. O. (2010). Wound healing effect of Flabellaria paniculata
leaf extracts. Journal of Ethnopharmacology, 127 (3), 786 – 788. https://doi.org/10.1016/j.
jep.2009.10.008
Pastar, I., Stojadinovic, O., Yin, N. C., Ramirez, H., Nusbaum, A. G., Sawaya, A., Patel, S. B., Khalid,
L., Isseroff, R. R., & Tomic-Canic, M. (2014). Epithelialization in wound healing: A
comprehensive review. Advances in Wound Care, 3 (7), 445 – 464. https://doi.org/10.1089/
wound.2013.0473
Rebolla, A., Arisawa, E. A. L. S., Barja, P. R., Posso, M. B. S., & Carvalho, C. D. S. (2013). Effect of
Brassica oleracea in rats skin wound healing. Acta Cirurgica Brasileira, 28 (9), 664 – 669.
https://doi.org/10.1590/S0102-86502013000900007
Reinke, J. M., & Sorg, H. (2012). Wound repair and regeneration. European Surgical Research, 49
(1), 35 – 43. https://doi.org/10.1159/000339613
Rex, J. R. S., Muthukumar, N. M. S. A., & Selvakumar, P. M. (2018). Phytochemicals as a potential
source for anti-microbial, anti-oxidant and wound healing – A review. MOJ Bioorganic
& Organic Chemistry, 2 (2), 61 – 70. https://doi.org/10.15406/mojboc.2018.02.0058
Rufai, Y., Isah, Y., & Isyaka, M. S. (2016). Comparative Phyto-Constituents Analysis from the
root bark and root core extractives of Cassia ferruginea (Schrad D. C) plant. Scholars
Journal of Agriculture and Veterinary Sciences, 3, 275 – 283. https://doi.org/10.21276/
sjavs.2016.3.4.1
Safna, M. I., Visakh, U. V., & Gangadharan, A. (2020). Biological activity of hexane extract of
Hemigraphis colorata, an indigenous wound healing plant. Materials Today, 25, 294 –
https://doi.org/10.1016/j.matpr.2020.01.461
Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K. M., & Latha, L. Y. (2011). Extraction, isolation
and characterization of bioactive compounds from plants’ extracts. African Journal
of Traditional, Complementary and Alternative Medicines, 8 (1), 1 – 10. https://doi.
org/10.4314/ajtcam.v8i1.60483
Sharif, M. K., Zahid, A., & Shah, F. H. (2018). Role of food product development in increased food
consumption and value addition. In A. M. Grumezescu, & A. M. Holban (Eds.), Food
processing for increased quality and consumption (pp. 455 – 479). Academic Press. https://
doi.org/10.1016/B978-0-12-811447-6.00015-1
Süntar, I., & Yakıncı, Ö. F. (2020). Potential risks of phytonutrients associated with high-dose or
long-term use. In S. M. Nabavi, & I. Suntar, D. Barreca, & H. Khan (Eds.), Phytonutrients in
food: From traditional to rational usage (pp. 137 – 155). https://doi.org/10.1016/B978-
-12-815354-3.00010-1
Tiwari, P., Kumar, B., Kaur, M., Kaur, G., & Kaur, H. (2011). Phytochemical screening and extraction: A review. Internationale Pharmaceutica Sciencia, 1, 98 – 106. https://doi.org/10.1002/hep.29375
Umeh, V. N., Ilodigwe, E. E., Ajaghaku, D. L., Erhirhie, E. O., Moke, G. E., & Akah, P. A. (2014). Woundhealing activity of the aqueous leaf extract and fractions of Ficus exasperata (Moraceae) and its safety evaluation on albino rats. Journal of Traditional and Complementary Medicine, 4 (4), 246 – 252. https://doi.org/10.4103/2225-4110.139105
Vitale, S., Colanero, S., Placidi, M., Di Emidio, G., Tatone, C., Amicarelli, F., & D’Alessandro, A. M. (2022). Phytochemistry and biological activity of medicinal plants in wound healing: An overview of current research. Molecules, 27 (11), 1 – 30. https://doi.org/10.3390/molecules27113566
Zhang, J., Fu, X., Li, W., Li, H., Ying, Z., Liu, X., & Yin, L. (2020). Enhancement of nutritional soy protein and peptide supplementation on skin repair in rats. Journal of Functional Foods, 75, 1 – 9. https://doi.org/10.1016/j.jff.2020.104231
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