Characterization of Lipase-Immobilized Polymethacrylate-based Monolith at Different Porogen Contents, Diameter, and Number of Holes for the Immobilization Process
Keywords:
Diameter, immobilization, lipase, polymethacrylate-based monoliths, porogen concentrationAbstract
Polymethacrylate-based monoliths have attracted a lot of attention due to their exceptional properties, such as reusability, solvent tolerance, and higher stability. This study investigates the physical characteristics and immobilization yield of lipase on polymethacrylate-based monoliths fabricated with varying porogen contents, hole diameters, and hole numbers. By systemically altering these structural parameters, this study aimed to elucidate their influence on monolith morphology, surface area, and pore architecture, which are critical for enzyme loading and catalytic performance. Immobilization yield was quantified via protein loading. Results revealed that the highest immobilization yield was achieved at 60% porogen concentration when varying the diameter and number of holes, with a yield of 58.67 ± 0.69%.At a diameter of 0.4 mm (18 holes) under varied porogen concentrations, immobilization yields of 57.50 ± 0.95% were obtained. In comparison, the simulation data predicted that the highest immobilization yield at a diameter of 0.3 mm (24 holes) with varied porogen concentration, yielding 55.90 ± 1.12% and immobilization yield of 58.90 ± 0.31% at 60% porogen content with different diameters and numbers of holes. These findings are crucial as they provide valuable insights into the design of tailored monolithic supports for biocatalytic applications, particularly in sustainable biodiesel production.
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