Antibiofilm Activity of Pseudomonas Sp. (Mb273n) Cell-Free Supernatant Against Pathogenic Bacteria
Keywords:
Bacterial biofilms, cell-free supernatant (CFS), biofilms of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureusAbstract
Bacterial biofilms pose a significant global health challenge due to their resistance to antibiotics and the host immune system, leading to persistent infections. Developing effective strategies to control biofilm formation is therefore crucial for preventing persistent infections. This study investigated the antibiofilm potential of the cell-free supernatant (CFS) from Pseudomonas sp. (MB273N) against biofilms of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Chromobacterium violaceum. Additionally, the study examined biofilm structural changes following CFS treatment. The CFS was obtained by centrifuging bacterial cultures three times at 11,000 rpm for 15 minutes. A biofilm inhibition assay was conducted to assess the antibiofilm activity of CFS at a sub-lethal concentration, and biofilm morphology was examined using scanning electron microscopy (SEM). Statistical analysis was performed using SPSS version 26. The CFS significantly (p<0.05) inhibited biofilm formation, with inhibition rates of 14.15% for E. coli, 32.46% for P. aeruginosa, 30.83% for S. aureus, and 37.5% for C. violaceum. SEM analysis revealed that untreated biofilms formed dense, multilayered structures, while CFS-treated biofilms appeared less dense, more dispersed, and exhibited altered cell morphology. The CFS of Pseudomonas sp. (MB273N) demonstrates potential as an antibiofilm agent against E. coli, P. aeruginosa, S. aureus, and C. violaceum, highlighting its possible application in biofilm control strategies.
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