The paralogous gene of myostatin deficiency does not improve the growth of Red seabream (Pagrus major)

Authors

  • Mitsuki OHAMA Aquaculture Research Institute, Kindai University, Shirahama 3153, Nishimuro, Wakayama 649-2211, Japan.
  • Kenta KISHIMOTO Division of Applied Bioscience, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto, 606-8502, Japan.
  • Masato KINOSHITA Division of Applied Bioscience, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto, 606-8502, Japan.
  • Keitaro KATO Aquaculture Research Institute, Kindai University, Shirahama 3153, Nishimuro, Wakayama 649-2211, Japan.
  • Youhei WASHIO Aquaculture Research Institute, Kindai University, Shirahama 3153, Nishimuro, Wakayama 649-2211, Japan

DOI:

https://doi.org/10.51200/bjomsa.v4i1.2445

Keywords:

Red seabream, CRISPR/Cas9, Myostatin, Muscle, Growth, Body shape

Abstract

To improve livestock and aquaculture-raised fish as food, targeted mutagenesis using genome editing technologies is becoming more realizable. Myostatin (Mstn), which functions as the negative regulator of skeletal muscle growth, is one of the major targets to improve the edible ratio of livestock and farmed fish. We previously reported that the deficiency of Pm-mstn, one of Myostatin paralogs, improves muscle growth and changes body shape in a finfish species, red seabream (Pagrus major), as a result of editing the gene by means of CRISPR/Cas9. In this study, we established Pm-mstnb-deficient red seabream, which is a null-allelic mutant of another paralogous gene of Myostatin in the species, and analyzed their phenotype in terms of growth traits and body shape. A comparison of all growth traits between Pm-mstnbwt/wt and Pm-mstnb-5/-5 revealed no significant differences. In addition, all metrics for body shape, defined as the ratios of body depth, body width, and depth of the caudal peduncle to body length, respectively, were also similar in Pm-mstnbwt/wt and Pm-mstnb-5/-5. Therefore, we concluded that Pm-mstnb does not function as a negative regulator of skeletal muscle growth in red seabream.

References

Ansai, S., Kinoshita, M. (2014). Targeted mutagenesis using CRISPR/Cas system in medaka. Biology Open 3, 362–371. https://doi.org/10.1242/bio.20148177

Brocal, I., White, R.J., Dooley, C.M., Carruthers, S.N., Clark, R., Hall, A., Busch-Nentwich, E.M., Stemple, D.L., Kettleborough, R.N.W. (2016). Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish. BMC Genomics 17, 259. https://doi.org/10.1186/s12864-016-2563-z

De Santis, C., Jerry, D.R. (2011). Differential tissue-regulation of myostatin genes in the teleost fish Lates calcarifer in response to fasting. Evidence for functional differentiation. Molecular and Cellular Endocrinology 335, 158–165. https://doi.org/10.1016/j.mce.2011.01.011

Gratacap, R.L., Wargelius, A., Edvardsen, R.B., Houston, R.D. (2019). Potential of genome editing to improve aquaculture breeding and production. Trends in Genetics 35, 672–684. https://doi.org/10.1016/j.tig.2019.06.006

Helterline, D.L.I., Garikipati, D., Stenkamp, D.L., Rodgers, B.D. (2007). Embryonic and tissue-specific regulation of myostatin-1 and -2 gene expression in zebrafish. General and Comparative Endocrinology 151, 90–97. https://doi.org/10.1016/j.ygcen.2006.12.023

Downloads

Published

2020-12-23

How to Cite

OHAMA, M., KISHIMOTO, K., KINOSHITA, M., KATO, K., & WASHIO, Y. (2020). The paralogous gene of myostatin deficiency does not improve the growth of Red seabream (Pagrus major). Borneo Journal of Marine Science and Aquaculture (BJoMSA), 4(1), 28–35. https://doi.org/10.51200/bjomsa.v4i1.2445

Issue

Vol. 4 No. 1 (2020): Borneo Journal of Marine Science and Aquaculture Vol. 4(1)/2020

Section

Articles
Crossref
Scopus
Google Scholar
Europe PMC
Total Views: 372 | Total Downloads: 264