RECOMBINANT PHYTASE: ADVANCES IN PRODUCTION STRATEGIES AND INDUSTRIAL APPLICATIONS – A REVIEW
Keywords:
Cell surface display of phytase, Phytase, Phytase production, Recombinant phytase.Abstract
As the demand for efficient and sustainable feed and food production continues to rise globally, phytase has become a vital enzyme in biotechnology due to its ability to hydrolyze phytic acid and enhance phosphorus bioavailability. Recent advances in genetic engineering, protein design, and molecular biology have significantly accelerated the development of high-yield and functionally enhanced phytase variants. A wide range of expression systems including bacterial, fungal, and yeast-based platforms have been extensively studied to improve catalytic efficiency, enzyme stability, and production yields under industrial conditions. Recombinant DNA technologies now allow precise modification of phytase genes for expression in intracellular, extracellular, or cell surface display formats, each offering unique advantages. Among these, cell surface display systems are gaining particular attention for their ability to anchor phytase directly onto microbial surfaces, simplifying purification and reducing downstream processing costs. Additionally, integration of synthetic biology tools, codon optimization, and promoter engineering has led to improved phytase production under harsh conditions such as high temperature or low pH. This review provides a comprehensive overview of current recombinant phytase production strategies, critically evaluating the advantages and limitations of various host systems and expression formats. Key factors influencing large-scale production are also discussed, with a focus on optimizing expression systems for industrial application. By addressing both technological innovations and production challenges, this review aims to support the development of more efficient, cost-effective, and environmentally sustainable phytase production platforms.
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