Genetic Diversity among Eight Species of Willow (Salix spp.) from Iran Based on SRAP Markers

Abstract

This study reports the application of sequence-related amplified polymorphism (SRAP) technique in characterization of 8 species of Salix (spp) from Iran by screening 22 primer combinations (PCs). Twenty two SRAP primer combinations could amplify 116 scorable loci, of which 107 bands were polymorphic. The amplified DNA fragments were used for calculation and statistical analysis. The Complete linkage cluster was performed and dendrogram drawn with the help of NTSYS pc 2.02 software which revealed two main clusters and several sub-clusters. This investigation showed that genetic distance was relatively significant among these species. The Jaccard similarity coefficient ranged from 0.18 to 0.55. The results also propose that the SRAP marker is a useful tool for evaluation of genetic diversity and relationships among different Salix species.