Preliminary Assessment of Secondary Metabolites of Selected Pteridophytes from Jorhat, Assam, India: Phytochemical Profiling of Assam Pteridophytes

Liza HANDIQUE; Baishali  BORAH; Amit  UPADHYAY; Mirzana AZAD

doi:10.51200/jtbc.v22i.5974

Authors

Liza HANDIQUE J.B. University
Baishali BORAH Dept. of Botany, Jagannath Barooah University, Jorhat, Assam, India
Amit UPADHYAY Dept. of Botany, Jagannath Barooah University, Jorhat, Assam, India
Mirzana AZAD

DOI:

https://doi.org/10.51200/jtbc.v22i.5974

Keywords:

Flavonoids, flavanols, phytochemical analysis, phenolic content, pteridophytes, traditional medicine

Abstract

This study analyses the phytochemical constituents of seven pteridophytic species from Assam, India. The methanolic extracts of seven species: Pyrrosia lanceolata, Pyrrosia nummularifolia, Ampheneuron opulentum, Sphenomeris chinensis, Selaginella sp., Diplazium esculentum, and Blechnum orientale were screened for phenolic, flavonoid, and flavonol content using standard spectrophotometric methods. Pyrrosia nummularifolia exhibited the highest flavonoid content, while Diplazium esculentum had the highest flavonol concentration. These bioactive compounds, known for their antimicrobial, antioxidant, and anti-inflammatory properties, support their traditional medicinal use and highlight their potential for drug discovery. Further studies are required to isolate and identify the pharmacologically active compounds from these species.

References

Adusei S, Otchere JK, Oteng P, Mensah RQ, Tei-Mensah E (2019) Phytochemical analysis, antioxidant and metal chelating capacity of Tetrapleura tetraptera. Heliyon 5(11): e02762. https://doi.org/10.1016/j.heliyon.2019.e02762.

Ainsworth EA, Gillespie KM (2007) Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature Protocols 2(4): 875–877. https://doi.org/10.1038/nprot.2007.102.

Amose PT, Sindhu N, Annas MM (2023) Phytochemical profiling and antibacterial potency of Blechnum orientale. The Journal of Indian Fern 40(1): 17–28.

Ayele DT, Akele ML, Melese AT (2022) Analysis of total phenolic contents, flavonoids, antioxidant and antibacterial activities of Croton macrostachyus root extracts. BMC Chemistry 16: 30. https://doi.org/10.1186/s13065-022-00822-0.

Borgohain P, Hazarika K (2020) Plant diversity with special reference to important medicinal plants in the Bhogdoi River of Jorhat, Assam, North East India. International Journal of Botany Studies 5(6): 736–742.

Borkotoky S, Sen S, Borah VV (2024) Assessment of bioactivity in four pteridophyte species from Assam, Northeast India, reveals prominent antioxidative and cytotoxic potential. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 94: 209–216. https://doi.org/10.1007/s40011-023-01526-8.

Borthakur S, Deka P, Nath KK (2000) The Illustrated Manual of Ferns of Assam. Bishen Singh Mahendra Pal Singh, Dehra Dun, India. 468 pp.

Chandra S, Fraser-Jenkins CR, Kumari A, Srivastava A (2008) A summary of the status of threatened pteridophytes of India. Taiwania 53(2): 170–209.

Choudhury J, Majumdar S, Roy S, Chakraborty U (2017) Antioxidant activity and phytochemical screening of two edible wetland pteridophytes Diplazium esculentum (Retz.) Sw and Marsilea minuta L. – A comparative study. World Journal of Pharmaceutical and Medical Research 3(9): 195–203.

Dutta H, Hazarika P, Hazarika K (2017) Wild edible plant species in patch vegetations of Jorhat district, Assam, India. International Research Journal of Biological Sciences 5(6): 2278–3202.

Fan Y, Feng H, Liu L, Zhang Y, Xin X, Gao D (2020) Chemical components and antibacterial activity of the essential oil of six Pyrrosia species. Chemistry and Biodiversity 17(4): e2000004.

Gogoi R, Behera S, Borah BC, Das SK, Nagesh TS, Kumar S, Deuri M (2023) Macrophyte diversity of Potiasola Beel, a closed floodplain wetland in Jorhat district of Assam. Journal of Experimental Zoology India 26(1): 1–10.

Goyal A, Middha S, Sen A (2010) Evaluation of the DPPH radical scavenging activity, total phenols and antioxidant activities in Indian wild Bambusa vulgaris ‘Vittata’ methanolic leaf extract. Journal of Natural Pharmaceuticals 1(1): 40–40.

Handique L (2024) Phytochemical analysis of methanolic extracts of leaves of some selected medicinal plants of Jorhat District, Assam, India. International Journal of Science and Research 13(3): 1–10.

Harborne JB (1998) Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Springer Science & Business Media, 302 pp.

Ho R, Teai T, Bianchini JP, Lafont R, Raharivelomanana P (2010) Ferns: From traditional uses to pharmaceutical development, chemical identification of active principles. In: Kumar A, Fernandez H, Revilla MA (eds.) Working with Ferns: Issues and Applications. Springer, New York. pp 321–346.

Ibadullayeva S, Movsumova NV, Shiraliyeva GS, Askerova NA, Mammadova HC (2022) Pteridophytes: Ethnobotanical use and active chemical composition. Indian Journal of Traditional Knowledge 21(2): 353–359.

Indriani IR, Hilwan YT, Hendra R (2023) Antioxidant and toxicological activities of Pyrrosia lanceolata (L.) Farw. extracts. Pharmacy Education 23(2): 174–178.

Ismiarni K, Azrifitria Y, Ofa S, Finti M, Maliyathun N (2015) Antioxidant and anti-inflammatory activity of the Indonesian ferns, Nephrolepis falcata and Pyrrosia lanceolata. International Journal of Pharmacy and Pharmaceutical Science 7(12): 162–165.

Kumaran A, Karunakaran J (2006) Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chemistry 97(1): 109–114.

Proestos C, Chorianopoulos N, Nychas GJ, Komaitis M (2005) RP-HPLC analysis of the phenolic compounds of fern extracts: Investigation of their antioxidant capacity and antimicrobial activity. Journal of Agricultural and Food Chemistry 53(4): 1190–1195.

Raja EF, Paul JJP (2019) Preliminary phytochemical and GC-MS analyses of methanolic extract of Blechnum orientale L. collected from Kothiyar, Kanyakumari District, Tamil Nadu, India. Journal of Drug Delivery and Therapeutics 9(4-A): 587–590.

Seifu T, Mehari B, Atlabachew M, Chandravanshi B (2017) Polyphenolic content and antioxidant activity of leaves of Urtica simensis grown in Ethiopia. Latin American Applied Research 40: 35–40.

Senguttuvan J, Paulsamy S, Karthika K (2014) Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. Asian Pacific Journal of Tropical Biomedicine 4(Suppl 1): S359–S367.

Sharma BD, Vyas MS (1985) Ethnobotanical studies on the fern and fern allies of Rajasthan. Bulletin of the Botanical Survey of India 27(1–4): 90–91.

Singleton LV, Rossi AJ (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 16(3): 144–158.