CYTOTOXICITY SCREENING AND ANTIBACTERIAL POTENTIAL OF BAKALI LEAF EXTRACT
DOI:
https://doi.org/10.51200/jbimpeagard.v3i2.1041Keywords:
antibacterial, Bakali, Artemia naupllii, S. aureus, E. coliAbstract
The study was conducted to screen the cytotoxic activity and to determine the antibacterial potential of the ethanol leaf extract of Bakali, a wild woody plant of the Bignoniaceae family and thrives on the slope of Mt. Arayat, Magalang, Pampanga. Four different concentrations (T1-10000 ppm, T2-1000 ppm, T3-100 ppm and T4-10 ppm), including T+ (ethanol) and T0 (saline solution) were prepared for cytotoxicity screening. Undiluted and diluted forms of the ethanol leaf extract, including T+ (chloramphenicol) and T- (distilled water) were utilized for antibacterial assay. Brine shrimps (Artemia naupllii) were used to determine the cytotoxic activity. The following percentages of deaths after nine hours of observation were recorded: T+ and T1 with 100%, T2 with 93.33%, and T3 with 3.33%. For T4 and T0, they had 0% deaths even after nine hours. The data also revealed that based on the Probit Method, the LC50, LC70 and LC95 of Bakali leaf extract to the brine shrimps after nine hours are expected to be at 363.35 ppm, 520.66 ppm, and 1122.95 ppm, respectively. On the other hand, Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli were used as test organisms for antibacterial assay using paper disc diffusion method. The results revealed that the ethanol leaf extract of Bakali had the potential to inhibit the growth of S. aureus and E. coli. Based on a 6-mm diameter paper disc, the extract is considered as “active†against S. aureus and “partially active†against E. coli when in the diluted form of aqueous solution.
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